Journal article
Wired for Success: Probing the Effect of Tissue-Engineered Neural Interface Substrates on Cell Viability
ATD Nascimento, AX Mendes, S Duchi, D Duc, LC Aguilar, AF Quigley, RMI Kapsa, DR Nisbet, PR Stoddart, SM Silva, SE Moulton
ACS Biomaterials Science and Engineering | Published : 2024
Abstract
This study investigates the electrochemical behavior of GelMA-based hydrogels and their interactions with PC12 neural cells under electrical stimulation in the presence of conducting substrates. Focusing on indium tin oxide (ITO), platinum, and gold mylar substrates supporting conductive scaffolds composed of hydrogel, graphene oxide, and gold nanorods, we explored how the substrate materials affect scaffold conductivity and cell viability. We examined the impact of an optimized electrical stimulation protocol on the PC12 cell viability. According to our findings, substrate selection significantly influences conductive hydrogel behavior, affecting cell viability and proliferation as a result..
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Grants
Awarded by Australian Research Council
Funding Acknowledgements
This work was supported by the Australian Research Council through the Centre of Excellence for Electromaterials Science (Project Number 140100012 CE) and the Aikenhead Centre for Medical Discovery - St Vincent's Hospital Melbourne. Four point probe analysis was performed at the Melbourne Centre for Nanofabrication (MCN) in the Victorian Node of the Australian National Fabrication Facility (ANFF) with the help of Dr. Abu Sadek. Illustrations in this manuscript were created using BioRender.com. D.R.N. acknowledges the Australian Research Council Future Fellowship project FT230100220.